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Short DescriptionCarbohydrate Labeling & Analysis KitDNA Size Standard Kit 600 bpLong Length Coolant Tubing Kit
Long DescriptionThe Carbohydrate Labeling and Analysis Kit is designed for use on the PA 800 Plus and contains the reagents, buffers, and capillaries required to label, separate, and quantify oligosaccharides and monosaccharides released from glycoproteins. After enzymatic or chemical release, sugars are labeled with a fluorophore (APTS) at the reducing termini by reductive amination. The stoichiometry of labeling is such that only one APTS molecule is attached to each molecule of oligosaccharide. These highly charged and fluorescent oligosaccharides are easily resolved in an electric field and detected by laser-induced fluorescence (LIF) detection. This kit provides reagent volumes for analysis of 100 samples and includes: • Carbohydrate Separation Buffer, 56 mL • N-CHO Coated Capillary, 2 units • Labeling Reagents, 1 vial • Labeling Dye (APTS), 4 x 5 mg • Labeling Dye Solvent, 1 mL • Glucose Ladder Standard, 50 mg • Quantitation/Mobility Marker (Maltose), 0.18 mg • APTS-M (monosaccharide-grade), 20 mgSize standards sufficient for 96 fragment analysis separations of fragments up to 600 nucleotides. This product includes mineral oil. The size standards included are DNA fragments of the following sizes labeled with WellRED fluorescent dye: 60, 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220, 240,260, 280, 300, 320, 340, 360, 380, 400, 420, 440, 460, 480, 500, 520, 540, 560, 580, 600, 620, and 640 nucleotides. Storage temperature: -20°C.Contains 90 cm, 74 cm, 68 cm, and 56 cm pre-formed coolant tubing lengths including nuts, ferrules, and o-rings for use with extended length capillaries or CE/MS methodologies.
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